Journal: Nature Communications
Article Title: Using rotational integration of oblique interferometric scattering to track axial spatiotemporal responses of tubular membrane protrusions
doi: 10.1038/s41467-026-72302-1
Figure Lengend Snippet: a i, Raw iSCAT image of 40 nm gold nanoparticles with 500 ms exposure time. Gold nanoparticles were dried thus adhered to the inner glass bottom, then PBS for 1:20 dilution to the initial storage liquid of nanoparticles. ii, Manually selected background pattern from PBS-only control dish under the same exposure time. iii, Final iSCAT image after post-processing of background subtraction. The dashed rectangle region highlights the blurring pattern in iSCAT, but is well-distinguished by RO-iSCAT. b i, RO-iSCAT images from different incoming azimuths without integration. ii, Final RO-iSCAT image with time-integrating during rotational scanning. c i, Intensity profile of raw image, background and the final result after subtraction, along the dashed line in magnified image cropped from ( a ) iii, the orange and green scatters are labelled by the left y axis while the blue curve is by right y axis. ii, Intensity profile of image at azimuth position of 0°, 90°, 180°, 270° and final integrated result along the dashed line in magnified image cropped from ( b ) ii, the blue curve is by right y axis while the scatters in other colours are labelled by left y axis. d Lateral and axial patterns captured from 40 nm gold particles, 100 nm polystyrene spheres and KPC extracellular vesicles, individually without rotational integration (top) and with rotational integration (bottom) configuration. Red dashed lines mark the region where xz cross-sections are shown. e Without rotational integration (top) against rotational integration (bottom) modality of CAF cells seeded with 40 nm nanoparticles. The magnifications highlight the tracking of membrane protrusions and nanoparticles. Scale bars : ( a , b , d ) 1 μm, ( c ) 500 nm, ( e ) 10 μm, insets in ( e ) 1 μm. Data shown are representative samples from 5 experiments for ( a , b , d ) and 3 experiments for ( e ).
Article Snippet: 40 nm gold nanoparticles were dried onto a glass bottom dishes (#1 coverslip thickness, CellVis) for 1 hr as described above.
Techniques: Control, Membrane
